Proteomic tool development for moss SUMOylome analysis.
Robert Augustine (Biology)
Project Type - B - Flexible: It will be in-person if we are allowed to have URSI students on campus, but it will become a remote project if not.
Small Ubiquitin-related Modifier (SUMO) is a post-translational modification that is rapidly attached to target proteins upon exposure to heat, cold, and drought and mitigates damage inflicted by these stresses. The identity of the SUMO targets in mosses are unknown. If ‘in-person’, the student will clone histidine-tagged SUMO constructs and introduce them into moss to enable purification of SUMO targets. The student will learn cloning, PCR, mutagenesis, Sanger Sequencing, and plant transformation and selection techniques. If ‘remote’, the student will mine plant genomes to facilitate identification of elusive SUMO pathway genes in moss. The information will be used to construct phylogenetic trees to infer evolutionary relationships and gene function. Students with programming skills are especially encouraged to apply for this project.
How should students express interest in this project? Interested students should briefly explain why they are interested in this research topic, and how it fits into their future goals. After reviewing applications, I will set up an interview with a short-list of compatible candidates, and then select from that group. Students should not contact me directly.
This is an 8-week project running from June 7 – July 31